Title : Photobiomodulation and Taurine in Human Neuroblastoma: In vitro study of Alzheimer's disease
The limited repair capacity of the central nervous system (CNS) is a viable challenge. Alzheimer's disease (AD) is a progressive and highly prevalent neurodegenerative disease that affects people worldwide. The complete inclusion of the CNS is not yet possible by means and is sought by means, including drug alternatives and LED irradiations to make neural tissue. In this work, we proposed establishing and standardizing an in vitro model of the use of hydrogen peroxide (H2O2) in a human neuroblastoma cell line (SH-SY5Y). Thus, the study explores the effects of taurine in conjunction with LED under oxidative stress to evaluate the neuroprotective effect and the ability of cellular restoration after oxidative stress. Recent research indicates that LED has excellent potential to be included in the treatment of AD, resulting in both therapies (LED and taurine supplementation), which may be promising. This work evaluated LED at wavelength at 660 nm and taurine as a pre-treatment and treatment model in oxidatively stressed cells, evaluating mitochondrial activity by the MTT colorimetric test (quantitative) and labeling of live cell mitochondria by fluorescence using MitoTracker Orange (qualitative). Cell viability was also evaluated by testing the blue cell structures by motive and cellular trypan (qualitative and quantitative). When performing as statistics and set as images, LED in standalone can present neuroprotective effects only in the pre-treatment, or analyzed, when the cell is exposed to LED and taurine and subsequently stressed with H2O2. Comparing the action of the two pre-treatments in contact with the cell and also in contact with cell + H2O2, it can be observed that LED has a more significant cell proliferative effect compared to taurine.
Keywords: Alzheimer's Disease; Neuroprotection; Oxidative stress.
What will audience learn from your presentation?
- With the presentation of our research, we intend to bring information about the applicability of photobiomodulation associated with drugs.
- Raise awareness of the benefits of photobiomodulation to the central nervous system (CNS).
- Provide subsidies for other works to be carried out associating photobiomodulation with cerebral revascularization, optimizing mitochondrial activity, potentiation of intracellular signaling, among others.